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Gel electrophoresis

Agarose Gel: Separating DNA by Size

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Learn how DNA moves through an agarose gel placed in a conductive buffer when a current is applied. Practise predicting the relative migration speeds of different-sized DNA fragments.

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Learning outcomes

  • Examine the movement of DNA through a conductive solution
  • Learn how an agarose gel placed in conductive buffer is able to separate DNA by size
  • Predict the relative migration speeds of various lengths of DNA travelling through an agarose gel
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Skills

Concepts, Principles and Theories

  • How the application of an electrical current affects the movement of DNA through the gel
  • Why the movement of DNA molecules through the gel is affected by their size
  • How DNA molecules of differing sizes move in relation to each other

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Dr Sheila Faherty
Altantic Technological University

"The simulations are brilliant reinforcing material, delivered at a good pitch with scope for elaboration and so clear and informative. Students could attempt them many times, and this really helped weaker students build their confidence."

Details

Degrees

Biomedical science
Biological science
Pharmacological science
Forensic science
Biochemistry

Modules

Molecular biology & genetics
Forensic investigation
Biological chemistry

Topic

Gel electrophoresis

Purchase option

Bioscience LabSim Library

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