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Gel electrophoresis

Agarose gel: troubleshooting potential errors

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This resource aims to help students understand potential errors in agarose gel electrophoresis for DNA analysis. It covers troubleshooting common issues related to sample loading, gel preparation and post-run analysis, enhancing their ability to interpret results and identify procedural errors.

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Learning outcomes

  • Deduce how agarose gels results are affected by experimental errors.
  • Assess validity in terms of DNA fragment presence and length for various gel results.
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Skills

Interpreting and Analysing Data

  • How to verify the presence of all DNA fragments
  • How to quantify the length of all DNA fragments

Concepts, Principles and Theories

  • How to determine the loading errors causing the results
  • How to determine the gel preparation error causing the result
  • How to determine the post-run error causing the result

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Dr. Alexandra Moores
University of Kent

"The Smart Worksheets are extremely useful to see the overall understanding and application of theories that we expected students to be able to use. Feedback is clear and accessible, enabling students to gain a deeper understanding of the topic they are studying and aiding in their success.”

Details

Degrees

Biomedical science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Gel electrophoresis

Purchase option

The Smart Worksheet Library

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