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Gel electrophoresis

Agarose gel: triplet repeat disease risk

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Apply agarose gel electrophoresis for diagnosing Triplet Repeat Diseases (TRD). Students use PCR, analyse gel results, construct a standard curve and interpret findings to assess disease risk.

Learning outcomes

  • Define experimental controls and components to be used for a given PCR-agarose scenario.
  • Construct a standard curve from a DNA ladder.
  • Calculate the number of repeats in a region of interest using standard curve and experimental results.
  • Evaluate how at risk the patient is to present symptoms of disease.
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Skills

Manipulating and Presenting Data

  • How to calculate the length of the repeat region and PCR amplicon for reference samples
  • How to calculate appropriate values and assemble a standard curve
  • What are appropriate axes titles and graph title for the standard curve
  • How to identify appropriate trendline for the standard curve
  • How to calculate the number of repeats in the TRD region for the patient sample and low-risk control

Using Instruments and Equipment

  • How to decide the contents and controls to run in each gel lane

Obtaining and Recording Data

  • How to record migration distances of ladder bands
  • How to record migration distances for control samples and patient sample

Concepts, Principles and Theories

  • How to select the appropriate DNA ladder for experimental scenarios

Interpreting and Analysing Data

  • What actions to take if the gel results are not as expected
  • How to identify the slope and y-intercept from the standard curve graph
  • How to determine the patient's disease risk based on calculated repeats
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Dr. Alexandra Moores
University of Kent

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Details

Degrees

Biological science
Biomedical science
Forensic science

Modules

Molecular biology & genetics

Topic

Gel electrophoresis

Purchase option

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