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Gel electrophoresis

Agarose gel: selecting appropriate time, voltage and concentration

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Explore how agarose gels are affected by time, voltage and agarose concentration to separate DNA fragments effectively. Students analyse gel images, identify errors and apply their knowledge in a realistic scenario.

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Learning outcomes

  • Deduce how agarose gel results are affected by time, agarose concentration and voltage.
  • Assess validity in terms of DNA fragment presence and length for various gel results.
  • Modify experimental setup to produce high quality results.
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Skills

Interpreting and Analysing Data

  • How to identify the ideal time, voltage and agarose concentration conditions to run effective gels
  • How to determine the usability of gel results for various purposes considering imperfect results

Concepts, Principles and Theories

  • How to use gels to to verify the presence of DNA
  • How to use gels to quantify DNA fragment length

Using Instruments and Equipment

  • How to adjust time, concentration and voltage to obtain optimal results
  • How to determine if your gel has run for the required time

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Balgisa, Pharmaceutical Sciences Student, London Metropolitan University

"I found using Smart Worksheets easy and clear. The lecturers helped us to understand how to work with them. They gave us a lot of examples and questions, so we practised a lot through our course. When the exam took place, we all were confident enough to pass this part of the exam."

Details

Degrees

Pharmacological science
Biological science
Biomedical science

Modules

Molecular biology & genetics
Forensic investigation

Topic

Gel electrophoresis

Purchase option

The Smart Worksheet Library

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