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Gel electrophoresis

Agarose gel: pathogen identification experiment

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This resource guides students through the application of agarose gel electrophoresis for pathogen identification. It covers experimental design, controls, data analysis and interpretation in a clinical context.

Learning outcomes

  • Define experimental controls and components to be used for a given PCR-agarose scenario.
  • Predict expected agarose gel band results based on a provided experimental set up.
  • Identify similarities and differences between expected and actual experimental results.
  • Conclude the identity of a pathogen based on agarose gel experimental results.
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Skills

Concepts, Principles and Theories

  • What the purpose of experimental controls is
  • How many experimental controls need to be run for a scenario
  • How to identify the appropriate DNA ladder for an experiment
  • How to identify the presence of a pathogen using gel electrophoresis

Interpreting and Analysing Data

  • How to determine if gels are giving the expected results from controls
  • How to match control bands with sample bands
  • How to determine the validity of results and what conclusions can be drawn

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Dr. Alexandra Moores
University of Kent

"The Smart Worksheets are extremely useful to see the overall understanding and application of theories that we expected students to be able to use. Feedback is clear and accessible, enabling students to gain a deeper understanding of the topic they are studying and aiding in their success.”

Details

Degrees

Biomedical science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Gel electrophoresis

Purchase option

The Smart Worksheet Library

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