< Back to the full list

Polymerase chain reaction (PCR)

Understanding qPCR

No items found.

Learn about incorporating quantification in PCR. Explore the principles of fluorescence and two different fluorescence detection methods used in qPCR. Understand the progression of a standard amplification plot and review a set of qPCR results.

Request a demo
See skills
arrow_downward

Learning outcomes

  • Review the fundamentals of PCR and its quantification
  • Identify the principles and differences of two fluorescence methods
  • Recognise the progression of a standard amplification plot
No items found.
chevron_left
chevron_right

Skills

Designing and Planning Experiments

  • How SYBR green and TaqMan probes differ in their specificity for DNA, interaction with DNA and cost
  • What are some of the key differences between endpoint PCR and qPCR

Interpreting and Analysing Data

  • What axes are used for an amplification plot 
  • How does the curve on an amplification plot reflect what is happening on a molecular level in the sample
  • How to use an amplification plot to compare the starting quantities of target DNA

Concepts, Principles and Theories

  • What are the 3 main stages of a PCR cycle and the temperatures at which they occur
  • What is meant by the term fluorophore
  • Why an increase in target DNA proportionally increases the fluorescence by SYBR green
  • Why an increase in target DNA proportionally increases the fluorescence by TaqMan probes 
  • Why the process is called qPCR or RT-PCR

Learn more about our approach to skills
Student response from Students Insights Survey

“I like that it goes through all the steps, so you have a better understanding of what you have to do for the actual lab... It makes me feel more prepared.”

Details

Degrees

Biochemistry
Biomedical science
Biological science
Pharmacological science
Forensic science

Modules

Biological chemistry
Molecular biology & genetics

Topic

Polymerase chain reaction (PCR)

Purchase option

Bioscience LabSim Library

Related products

Polymerase chain reaction (PCR)

PCR primer design

Smart Worksheet

Polymerase chain reaction (PCR)

Running a Polymerase Chain Reaction (PCR)

LabSim

Polymerase chain reaction (PCR)

Key Stages in a Polymerase Chain Reaction (PCR)

LabSim
toggle video playback

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page
toggle video playback

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope