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Polymerase chain reaction (PCR)

Key Stages in a Polymerase Chain Reaction (PCR)

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Learn about the key steps involved in a polymerase chain reaction. Explore each stage of a PCR cycle: denaturation, primer annealing, and primer extension.

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Learning outcomes

  • Explore, in detail, the stages of one PCR cycle
  • Answer questions on the theory of PCR
  • Consider the power of DNA amplification by doubling
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Skills

Concepts, Principles and Theories

  • What happens during one cycle of amplification
  • What reagents are required to carry out PCR
  • How changes in temperature initiate the different phases of PCR
  • How multiple cycles of PCR can be used to amplify a region of DNA

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Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."

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Degrees

Biochemistry
Biological science
Biomedical science
Forensic science
Pharmacological science

Modules

Biological chemistry
Molecular biology & genetics
Forensic investigation

Topic

Polymerase chain reaction (PCR)

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