< Back to the full list

Polymerase chain reaction (PCR)

PCR primer design

No items found.

Explore the fundamental principles of an effective PCR primer such as primer orientation, primer length, primer melting temperature and restriction enzyme sites. Then design your own primers to amplify a target region. Study amplicon to determine restriction enzyme sites and design primer extensions that can be used for vector insertion.

Request a demo
See skills
arrow_downward

Learning outcomes

  • Identify characteristics of forward and reverse primer binding to DNA.
  • Determine how primer length, melting temperature and GC content influence DNA amplification.
  • Design a set of primers to amplify a sequence of interest.
  • Determine which restriction endonucleases to use for cloning a target DNA fragment.
No items found.
chevron_left
chevron_right

Skills

Designing and Planning Experiments

  • Use the key principles of PCR to design primers
  • Write DNA sequences of primers 
  • Design primer extensions

Concepts, Principles and Theories

  • How primers are affected by length, melting temperature and GC content
  • How restriction enzymes create overhang regions

Learn more about our approach to skills
Student, University of Brighton, Pharmacy and Biomolecular Sciences

"The Smart Worksheet has been excellent; it's helped so much to get feedback as I go through and I don't know how I would have managed the coursework otherwise!"

Details

Degrees

Biochemistry
Biomedical science
Biological science
Pharmacological science
Forensic science

Modules

Molecular biology & genetics

Topic

Polymerase chain reaction (PCR)

Purchase option

The Smart Worksheet Library

Related products

Polymerase chain reaction (PCR)

Understanding qPCR

LabSim

Polymerase chain reaction (PCR)

Running a Polymerase Chain Reaction (PCR)

LabSim

Polymerase chain reaction (PCR)

Key Stages in a Polymerase Chain Reaction (PCR)

LabSim
toggle video playback

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page
toggle video playback

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope