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Molecular cloning

Plasmid Ligation

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Learn about the catalysis of a ligation reaction by DNA ligase, and explore the benefits of using phosphatase treatment and double digests to prevent the religation of plasmids.

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Learning outcomes

  • See how DNA ligase catalyses a ligation reaction
  • Consider the benefits of using phosphatase treatment before ligation
  • Look at the use of double digests to prevent plasmid religation
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Skills

Designing and Planning Experiments

  • What reagents and cofactors are required for the ligation reaction 
  • How to select the most suitable restriction enzymes for a double digest

Concepts, Principles and Theories

  • What is happening on a molecular level during the digestion and ligation reaction 
  • How the use of phosphatase or multiple restriction enzymes can prevent religation of the plasmid DNA

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Prof. Mike Davies-Coleman
University of the Western Cape

"We’ve been bringing the labs to our students online, allowing them to immerse themselves in a user-friendly interactive space, learn the relevant skills and feed that back into their future experiments. As a result of using LabSims to enhance our teaching practice, student grades increased."

Details

Degrees

Biological science
Biomedical science
Pharmacological science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Molecular cloning

Purchase option

Bioscience LabSim Library

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