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Molecular cloning

Miniprep 2: Purify Plasmid DNA

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Learn the key steps involved in purifying plasmid DNA in a miniprep protocol. Test your knowledge and understanding with interactive animations and questions.

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Learning outcomes

  • Use a spin column with a silica membrane to bind the plasmid DNA
  • Homogenise the lysate and add it to a silica spin column
  • Wash the column to remove impurities
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Skills

Using Instruments and Equipment

  • How the features of a miniprep column facilitate the purification of plasmid DNA

Obtaining and Recording Data

  • Which buffers are required to wash the membrane and elute the plasmid DNA 
  • Why it is important to wash the membrane

Concepts, Principles and Theories

  • What is happening on a molecular level during each step of the purification process

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Dr Sheila Faherty
Altantic Technological University

"The simulations are brilliant reinforcing material, delivered at a good pitch with scope for elaboration and so clear and informative. Students could attempt them many times, and this really helped weaker students build their confidence."

Details

Degrees

Biological science
Biomedical science
Pharmacological science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Molecular cloning

Purchase option

Bioscience LabSim Library

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