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Molecular cloning

Cloning and Expression Plasmids

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Learn about the key elements of cloning and expression plasmids. Explore the process of transcription and translation of a gene of interest inserted into an expression plasmid, and practise designing suitable cloning and expression plasmids for experiments.

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Learning outcomes

  • Label the different parts of a cloning plasmid
  • Correctly order the parts of an expression plasmid
  • Read through a set of cloning scenarios, and design a plasmid suitable for each experiment
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Skills

Designing and Planning Experiments

  • What experimental factors must be considered when designing an appropriate plasmid

Concepts, Principles and Theories

  • How different elements found in cloning plasmids facilitate the insertion, replication and selection of DNA
  • How components within an expression plasmid facilitate the transcription and translation of a protein of interest

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Dr Anna Maria Piccinini
University of Nottingham

"Always accessible everywhere, these resources have added flexibility to PGR training, allowing students to safely prepare for the lab and regain self-confidence"

Details

Degrees

Biomedical science
Biological science
Pharmacological science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Molecular cloning

Purchase option

Bioscience LabSim Library

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