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Molecular cloning

Miniprep 1: Isolate Plasmid DNA

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Learn the key steps involved in isolating plasmid DNA in a miniprep protocol. Test your knowledge and understanding with interactive animations and questions.

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Learning outcomes

  • Collect bacterial cells containing plasmid
  • Break open cells to release their contents
  • Isolate plasmid DNA from other cell contents using the alkaline lysis method
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Skills

Obtaining and Recording Data

  • Which reagents and processes are required to isolate plasmid DNA 
  • When to discard the supernatant and when to discard the pellet 
  • Why it is important to thoroughly mix the contents following resuspension, lysis and neutralisation steps
  • When it is appropriate to vortex samples and when they should be gently inverted

Concepts, Principles and Theories

  • What is happening on a molecular level during centrifugation, resuspension, lysis and neutralisation steps

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University of the Western Cape

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Details

Degrees

Biomedical science
Biological science
Pharmacological science
Forensic science
Biochemistry

Modules

Molecular biology & genetics

Topic

Molecular cloning

Purchase option

Bioscience LabSim Library

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