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Cloning

Miniprep 1: isolate plasmid DNA

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Learn the key steps involved in isolating plasmid DNA in a miniprep protocol. Test your knowledge and understanding with interactive animations and questions.
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Learning outcomes

  • Collect bacterial cells containing plasmid.
  • Break open cells to release their contents.
  • Isolate plasmid DNA from other cell contents using the alkaline lysis method.
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Skills

Obtaining and Recording Data

  • Which reagents and processes are required to isolate plasmid DNA 
  • When to discard the supernatant and when to discard the pellet 
  • Why it is important to thoroughly mix the contents following resuspension, lysis and neutralisation steps
  • When it is appropriate to vortex samples and when they should be gently inverted

Concepts, Principles and Theories

  • What is happening on a molecular level during centrifugation, resuspension, lysis and neutralisation steps

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Prof. Mike Davies-Coleman
University of the Western Cape

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Details

Degrees

Biological science
Biomedical science
Foundation science

Discipline

Molecular biology

Topic

Cloning

Purchase option

Bioscience LabSim Library

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Biological science
Biomedical science
Foundation science
cloning
Molecular biology
Bioscience LabSim Library
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