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Genotyping

SSLP Detection

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Learn about Simple Sequence Length Polymorphisms (SSLPs) and how to use PCR and agarose gel electrophoresis to isolate and visualise them. Practise predicting the size of PCR products for different alleles, identifying band patterns and creating an SSLP detection workflow.

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Learning outcomes

  • Define SSLP
  • Predict PCR products of alleles with SSLP
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Skills

Concepts, Principles and Theories

  • What is meant by simple sequence length polymorphisms (SSLP’s)
  • How an SSLP affects the length of the expected PCR products

Interpreting and Analysing Data

  • How to identify the alleles present in different DNA samples using PCR and agarose gel electrophoresis

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Prof. Mike Davies-Coleman
University of the Western Cape

"We’ve been bringing the labs to our students online, allowing them to immerse themselves in a user-friendly interactive space, learn the relevant skills and feed that back into their future experiments. As a result of using LabSims to enhance our teaching practice, student grades increased."

Details

Degrees

Biochemistry
Biomedical science
Biological science
Pharmacological science
Forensic science

Modules

Molecular biology & genetics

Topic

Genotyping

Purchase option

Bioscience LabSim Library

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