< Back to the full list

Nucleic acid quantification

DNA Quantification

No items found.

Learn how to compare and use NanoDrop and Qubit technology, explore DNA, salt and protein spectra, and measure DNA using a fluorometer. Understand the pros and cons of each technology and interpret the results.

Request a demo
See skills
arrow_downward

Learning outcomes

  • Explain how DNA can be quantified and contamination detected using a NanoDrop
  • Explain how DNA can be quantified using a Qubit
  • Outline the pros and cons of each method
No items found.
chevron_left
chevron_right

Skills

Designing and Planning Experiments

  • How the NanoDrop and Qubit differ in terms of sample preparation, type of measurement and accuracy

Interpreting and Analysing Data

  • How to interpret a typical absorbance spectra and results from a NanoDrop
  • What 260/280 and 230/260 ratios are indicative of significant contamination

Concepts, Principles and Theories

  • Why the absorbance at 260 nm is used to measure DNA concentration 
  • Why the 260/280 and 260/230 ratios indicate the level of protein and salt contamination
  • Why quantification of dsDNA using a fluorometer relies on the addition of a fluorescent dsDNA binding dye

Learn more about our approach to skills
Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."

Details

Degrees

Biochemistry
Biomedical science
Pharmacological science
Forensic science
Biological science

Modules

Molecular biology & genetics
Forensic investigation

Topic

Nucleic acid quantification

Purchase option

Bioscience LabSim Library

Related products

Crime scene investigation

Incident Scene: assessment and examination

Smart Worksheet

Crime scene investigation

Scene management: prior to the examination

Smart Worksheet

Nucleic acid extraction

RNA Extraction from Animal Cells

LabSim

Sequencing

Next Generation Sequencing

LabSim
toggle video playback

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page
toggle video playback

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope