Organ bath

Organ Bath - Ileum

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Learn about how an organ bath works and how it can be used to determine the effect of drugs on an isolated piece of aorta. Practise using the equipment to wash a piece of tissue and determine the effect of 4 drugs, including acetylcholine and histamine on the ileum. Analyse a force-time trace to identify the effect of each drug on the tissue.

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Learning outcomes

Consider the role of different parts of the organ bath equipment
Determine the effect of four different drugs on ileum
Relate the shape of the force-time trace to the underlying pharmacology

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Skills

Using Instruments and Equipment

What are the different parts of the organ bath equipment
How to run a single wash cycle

Obtaining and Recording Data

Why the tissue must be washed in between drug applications
How to annotate the force-time trace generated by the chart recorder

Interpreting and Analysing Data

What receptors are bound by acetylcholine, pilocarpine, mepyramine and histamine
How to determine whether a drug causing smooth muscle contraction by analysing a force-time trace

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Prof. Gavin Maneveldt

University of the Western Cape

Having more time to spend on the actual practicals showed a great improvement in the marks achieved by students as well. The positive interactions with the students demonstrated that they had engaged well with the online resources.

Details

Degrees

Biochemistry

Pharmacological science

Biomedical science

Modules

Pharmacology

Topic

Organ bath

Purchase option

Bioscience LabSim Library

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