Light microscopy

Kohler Illumination

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Learn about Köhler illumination and the benefits of using this technique. Familiarise yourself with the components of a microscope and how to adjust the light source for optimal imaging. Practise adjusting the diaphragm and condenser to achieve optimal contrast and resolution.

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Learning outcomes

Learn why it is important to set a microscope up for Köhler illumination
Familiarise yourself with the parts of a microscope that need to be adjusted to establish Köhler illumination
Work through the steps involved in configuring a microscope for Köhler illumination

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Skills

Using Instruments and Equipment

What are the steps involved in setting up a microscope for Köhler illumination
What is happening mechanically when the field diaphragm is adjusted
How does adjusting the field diaphragm affect the image seen down the microscope
What is happening mechanically when the condenser height is adjusted
How does adjusting the height of the condenser affect the image seen down the microscope
What is happening mechanically when the condenser centering screws are adjusted
How does adjusting the centering screws on the condenser affect the image seen down the microscope
What is happening mechanically when the iris diaphragm is adjusted
How does adjusting the iris diaphragm affect the image seen down the microscope

Concepts, Principles and Theories

What it means to set a microscope up for Köhler illumination

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Light microscopy

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