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Light microscopy

Focusing and Navigating

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Learn how to use microscope focus controls to bring an image into focus at 3 different magnifications, and make appropriate adjustments to the brightness with each different objective lens. Practise using the stage controls to bring an area of interest into the centre.

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Learning outcomes

  • Use the focus controls to bring an image into sharp focus at 3 different magnifications
  • Explore the concept of depth of focus and how this changes with increasing magnification
  • Familiarise yourself with the controls used to move the microscope stage and use these to centre the field of view on a region of interest
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Skills

Using Instruments and Equipment

  • How to adjust the coarse and fine focus controls to bring an image into sharp focus
  • Why the magnification should be increased incrementally 
  • Why some objective lens’ require the use of immersion oil 
  • How to apply immersion oil 
  • How to adjust the brightness to increase image clarity 
  • How to use the stage controls to bring an area of interest into the centre of the field of view

Obtaining and Recording Data

  • Why care must be taken when raising the stage 
  • Why the coarse focus control should only be used on low power objectives 

Concepts, Principles and Theories

  • How the level of magnification affects the depth of focus 
  • How the orientation of the image seen down the microscope compares to the orientation of the sample on the slide

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Dr Sheila Faherty
Altantic Technological University

"The simulations are brilliant reinforcing material, delivered at a good pitch with scope for elaboration and so clear and informative. Students could attempt them many times, and this really helped weaker students build their confidence."

Details

Degrees

Biochemistry
Biological science
Biomedical science
Forensic science
Pharmacological science

Modules

Anatomy & physiology
Tissue & cell biology
Microbiology
Ecology & evolution
Immunology
Forensic investigation

Topic

Light microscopy

Purchase option

Bioscience LabSim Library

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