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Histology

Histological staining

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This Smart Worksheet is a guide to histological staining, covering tissue preparation, the Hematoxylin and Eosin (H&E) staining method and error analysis through visual assessment of stained specimens. Students will gain theoretical knowledge of staining procedures and practical skills, troubleshooting common staining problems.

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Learning outcomes

  • Describe the purpose and effects of histological staining at various steps.
  • Describe the components and reagents of histological staining.
  • Specify the reagents used at various steps in histological staining.
  • Identify and amend errors in histological staining.
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Skills

Concepts, Principles and Theories

  • What the steps in histological staining are and their purpose
  • How to differentiate between different staining methods
  • How each of the different reagents is used to contribute to the staining of different cellular parts

Using Instruments and Equipment

  • How to use each of the reagents and solutions in an H&E stain

Interpreting and Analysing Data

  • How to identify good and poor results for an H&E stain
  • How to identify specific problems with a stain and adjust to fix errors

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Dr. Alexandra Moores
University of Kent

"The Smart Worksheets are extremely useful to see the overall understanding and application of theories that we expected students to be able to use. Feedback is clear and accessible, enabling students to gain a deeper understanding of the topic they are studying and aiding in their success.”

Details

Degrees

Biological science
Biomedical science

Modules

Bio essentials
Tissue & cell biology
Microbiology

Topic

Histology

Purchase option

The Smart Worksheet Library

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