Histology

Haematoxylin & Eosin (H&E) Stain

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Learn about the reagents, mechanism and steps involved in a principal histological stain. Explore the mechanism of staining by the two dyes involved in the process, and rearrange the steps of the protocol to optimise the staining. Identify the correct order of reagents and the role of certain reagents in the protocol.

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Learning outcomes

Describe the mechanism of staining by the two dyes
List the order and role each reagent plays in the staining protocol
Troubleshoot the protocol to optimise staining

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Skills

Concepts, Principles and Theories

What is the purpose of a H&E stain
Which cellular structures are stained by Haematoxylin and Eosin
What is the role of the different reagents used in a H&E stain

Using Instruments and Equipment

How to prepare the tissue for staining
How to stain the tissue with H&E
How to prepare the tissue for coverslipping
Why the solutions for each phase of the staining process must be applied in a specific order

Obtaining and Recording Data

What effect altering the time in the staining solutions has on the quality of the final result
How best to adjust the protocol to correct for some common issues with the H&E stain

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Details

Degrees

Biochemistry

Biological science

Biomedical science

Forensic science

Pharmacological science

Modules

Tissue & cell biology

Anatomy & physiology

Topic

Histology

Purchase option

Bioscience LabSim Library

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