Cytometry

Flow Cytometry: Fundamentals

No items found.

Learn about the internal components of a flow cytometer. Explore how differences in the morphology and protein expression of cells affect the light signals generated and allow for the characterisation of cell populations. Practise identifying and gating cell populations from dot plots.

Request a demo

See skills

arrow_downward

Learning outcomes

Explore the different components of a flow cytometer and how light signals are collected
Understand the different parameters that can be analysed using flow cytometry
Familiarise yourself with common data presentation and data analysis strategies

No items found.

chevron_left

chevron_right

Skills

Designing and Planning Experiments

How to configure the optics to split out light of the desired wavelength

Using Instruments and Equipment

How the laser, fluidics, optics, detectors and electronics of a flow cytometer together measure light signals
What types of light signal are measured by a flow cytometer and what information they provide

Concepts, Principles and Theories

How scattered and fluorescent light signals are generated and recorded
What is meant by forward scatter, side scatter and granularity
How the size and granularity of a cell affect the scattered light signals

Interpreting and Analysing Data

How to interpret dot plots to distinguish between cell populations
How gating can be used to compare many different parameters and isolate populations of interest

Learn more about our approach to skills

Prof Nicky King

University of Exeter

“Over 95% [of students] said they’d recommend them to a friend and that they wanted these resources across all the modules they were studying. So, they clearly had a really positive impact on the student experience.”

Details

Degrees

Biomedical science

Biological science

Modules

Immunology

Topic

Cytometry

Purchase option

Bioscience LabSim Library

Related products

Quantitative skills

Fundamental numeracy (Biomed)

Smart Worksheet

Cell culture

Cell Counting: Haemocytometer

LabSim

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.

Enhance student skills and accelerate learning with multiple opportunities to practise
Boost confidence and competency with enhanced support and personalised feedback
Engage and motivate with enjoyable, challenging learning experiences

LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice

Improve the student experience of assessment with immediate, targeted feedback
Save academic time by minimising repetitive marking for staff
Gain immediate insight into student progress with individual performance data

Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.

Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

Try our resources for free
Get a live demonstration of our products
Become a partner and join our community
Talk to our team of experts

Request a demo

Image of a smiling student with a microscope