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Cell culture

Cell Counting: Haemocytometer

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Learn how to use a Neubauer haemocytometer to perform a viable cell count. Practise assembling the equipment, preparing and loading the sample, counting the number of cells and calculating the viable cell density and percentage viability.

Learning outcomes

  • Prepare and load the sample into the haemocytometer
  • Count the number of cells in a sample
  • Manipulate the data to calculate cell density and cell viability
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Skills

Using Instruments and Equipment

  • How a haemocytometer can be used to count cells
  • How to affix the coverslip 
  • How to load the sample 
  • How to count cells

Obtaining and Recording Data

  • Why it is important to clean the equipment prior to counting
  • Why care must be taken when loading the sample into the chamber
  • Why it is important to mix the sample prior to counting
  • How trypan blue can be used to differentiate between viable and non-viable cells

Manipulating and Presenting Data

  • How to calculate the viable cell density of the original sample
  • How to calculate the percentage viability of the original sample

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Dr Samuel Egieyeh
University of the Western Cape

"The Learning Science platform, with its interactive animations, provides the students with a basic understanding of the central dogma of molecular biology and provides the link between the basic biotechnology experiments and the complex production process, formulation, and quality control of biologics."

Details

Degrees

Biomedical science
Biological science
Pharmacological science
Forensic science
Biochemistry

Modules

Anatomy & physiology
Tissue & cell biology
Immunology
Pharmacology

Topic

Cell culture

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