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Protein assays

Protein purification and yield

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Perform calculations involved in protein purification. Analyse experimental results from hypothetical students, calculating concepts like total/specific activity, percent yield and purification factor.

Learning outcomes

  • Define 'total activity', 'percent recovery', 'specific activity' and 'purification factor'.
  • Calculate the total activity and the percent recovery for a cell extract and purified sample.
  • Calculate the specific activity and the purification factor for a cell extract and purified sample.
  • Evaluate experimental purification data to determine relative success.
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Skills

Concepts, Principles and Theories

  • How to apply total activity and specific activity in context
  • How to differentiate percent recovery and purification factor
  • How to determine level of success of purification for outlined objective

Interpreting and Analysing Data

  • How to calculate total activity and specific activity
  • How to calculate percent recovery from crude product
  • How to calculate purification factor of extracted product

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Balgisa, Pharmaceutical Sciences Student, London Metropolitan University

"I found using Smart Worksheets easy and clear. The lecturers helped us to understand how to work with them. They gave us a lot of examples and questions, so we practised a lot through our course. When the exam took place, we all were confident enough to pass this part of the exam."

Details

Degrees

Pharmacological science
Biochemistry
Biomedical science
Biological science

Modules

Molecular biology & genetics
Tissue & cell biology

Topic

Protein assays

Purchase option

The Smart Worksheet Library

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