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pH & buffers

Making a Buffer

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Understand how to create a buffer solution at a specific pH from stock solutions. Calculate the concentration and moles of a weak acid or base and conjugate in the resulting buffer, and calculate the volumes of stock solutions required.

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Learning outcomes

  • Rearrange pH equationRearrange the Ka equation
  • Determine the volumes of weak acid and conjugate base to take from stock solutions in order to make a buffer solution of desired pH
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Skills

Concepts, Principles and Theories

  • How to apply and rearrange the pH and pKa equations.

Manipulating and Presenting Data

  • How to calculate the proton concentration from the pH 
  • How to calculate the relative concentration of A- and HA from the Ka and proton concentration  
  • How to determine the relative moles of A- and HA from the relative concentration 
  • How to calculate the relative volumes of A- and HA to take from stock solutions
  • How to determine the actual volumes of A- and HA to take from stock solutions 

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Designing and Planning Experiments

  • How to calculate the required volumes of weak acid and conjugate base needed to make a buffer solution of desired pH

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University of Glasgow

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Degrees

Biochemistry
Biomedical science
Biological science
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Chemistry

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Biological chemistry
Preparative chemistry
Quantitative chemistry

Topic

pH & buffers

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