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Characterising molecules

Mass spectrometry fundamentals

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An interactive exploration of mass spectrometry fundamentals from familiarisation with how mass spectrometers work, interoperation of fragmentation patterns, to analysis of spectra and apply understanding of ionisation techniques to determine molecular structures.

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Learning outcomes

  • Identify the order of key steps of running a mass spectrometer.
  • Summarise concepts about fragmentation and separation of particles during mass spectroscopy.
  • Identify fragment ions and their lost radical using mass to charge ratio (m/z).
  • Interpret a mass spectrum by identifying (m/z) of molecular ion, base peak and potential radical loss.
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Skills

Concepts, Principles and Theories

  • How a mass spectrometer works to achieve fragmentation of a molecule
  • How to identify lost radicals and their effect on mass spectrometry data

Using Instruments and Equipment

  • What the required steps are to run a mass spectrometer

Interpreting and Analysing Data

  • How to identify the structure of a fragment ion based on its m/z value
  • How to determine m/z value of the base peak from a spectrum
  • How to determine m/z value for molecular ion peak

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Student, University of Glasgow

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Details

Degrees

Chemistry
Forensic science
Pharmacological science
Biochemistry
Biomedical science

Modules

Biological chemistry
Pharmacology
Analytical chemistry

Topic

Characterising molecules

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