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Gel electrophoresis

Loading and Running an SDS-PAGE Gel

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Learn about the steps involved in loading and running an SDS-PAGE gel. Practise loading protein samples, adjusting power pack settings and processing the gel by choosing how best to trim it using a wedge tool.

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Learning outcomes

  • Familiarise yourself with the appearance of an SDS gel tank
  • Load a protein sample into an SDS-PAGE gel
  • Set up equipment for running an SDS-PAGE gel
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Skills

Using Instruments and Equipment

  • What are the main features of the set-up once the gel is placed in an electrophoresis tank 
  • How to position the pipette tip to carefully deposit the sample in the well of the gel 
  • What are the typical components of the sample loading buffer 
  • Why it is important to correctly wire the electrodes to the tank
  • Where the gel should be trimmed after it has finished running

Obtaining and Recording Data

  • Why a protein ladder is loading alongside the samples 
  • How the voltage selected can affect the running of the gel and the quality of the results

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Coventry University

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Details

Degrees

Biochemistry
Biological science
Biomedical science
Forensic science
Pharmacological science

Modules

Biological chemistry

Topic

Gel electrophoresis

Purchase option

Bioscience LabSim Library

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