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Identification of microorganisms

Gram Stain

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Familiarise yourself with the differences in the cell wall composition of Gram negative and Gram positive bacteria. Explore the four main reagents used in a Gram stain and how the Gram stain process differentiates between these two types of bacteria.

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Learning outcomes

  • Familiarise yourself with the differences in the cell wall composition of Gram negative and Gram positive bacteria
  • Learn the four main reagents used in a Gram stain and their effect within cells
  • Understand how the Gram stain process differentiates between these two types of bacteria
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Skills

Concepts, Principles and Theories

  • What is the purpose of a Gram stain
  • How the cell wall of Gram positive and Gram negative bacteria differs
  • What four solutions are used to perform a Gram stain
  • What effect each solution has on the cell wall of Gram positive and negative bacteria 
  • What colour Gram positive and negative bacteria appear after the application of each solution
  • How the Gram stain relies on the differences in the cell walls of Gram positive and negative bacteria

Using Instruments and Equipment

  • What order the four solutions are applied
  • How to apply each solution 
  • How to perform a wash step 

Obtaining and Recording Data

  • What effect adding too much or too little of each solution has on the final result 

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Details

Degrees

Biochemistry
Biomedical science
Biological science
Forensic science
Pharmacological science

Modules

Microbiology

Topic

Identification of microorganisms

Purchase option

Bioscience LabSim Library

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